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quantstudio 3d digital pcr chip loader  (Thermo Fisher)


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    Structured Review

    Thermo Fisher quantstudio 3d digital pcr chip loader
    Quantstudio 3d Digital Pcr Chip Loader, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantstudio+3d+digital+pcr+chip+loader/pmc11449942-260-28-36
    Average 90 stars, based on 1 article reviews
    quantstudio 3d digital pcr chip loader - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Digital PCR:

    Article Title: Haemagglutinin substitutions N125D, D127E, D222G and R223Q improve replicative fitness and vaccine effectiveness of an A/H1N1pdm09 live attenuated influenza vaccine virus by enhancing α-2,6 receptor binding
    Article Snippet: 14.5μL dPCR reactions were then set up using 5μL cDNA at a range of 10-fold dilutions (neat, 1 x 10 −1 –1 x 10 −6 ) to identify the dilution within ‘digital’ quantification range. cDNA was combined with 200nM of the M13 forward primer (GTAAAACGACGGCCAG), 800nM of the gene specific HA reverse primer (GAAATGGGAGGCTGGTG), 100nM of the gene specific hydrolysis probe (FAM/GGACACTAG/ZEN/TAGAGCCGGGA/BHQ), 7.25μL QuantStudio 3D Digital PCR Master Mix V2 (Thermo Fisher, Cat. No. A26358) and made up to the final volume of 14.5μL with water. .. The dPCR reactions were loaded onto chips as described in the manufacturer’s protocol using the QuantStudio 3D Digital PCR Chip Loader with QuantStudio 3D Digital PCR 20K Chip Kit V2 (Thermo Fisher, Cat. No. A26316). ..

    Article Title: Extraction-Free Absolute Quantification of Circulating miRNAs by Chip-Based Digital PCR
    Article Snippet: .. Chip-based dPCR was performed on a QuantStudio 3D Digital PCR System platform composed of the QuantStudio 3D Instrument (Thermo Fisher Scientific; 4489084), the Dual Flat Block GeneAmp PCR System 9700 (Thermo Fisher Scientific; 4484078), and the QuantStudio 3D Digital PCR Chip Loader (Thermo Fisher Scientific; 4482592). dPCR was performed according to the manufacturer’s instructions. ..

    Article Title: Mitochondrial aggregation caused by cytochalasin B compromises the efficiency and safety of three-parent embryo.
    Article Snippet: .. The sample was loaded using the QuantStudio 3D Digital PCR Chip Loader (4482592, Thermo Fisher Scientific, Singapore) and amplified in a GeneAmp PCR System 9700. .. Images of chips were taken using the QuantStudio 3D Digital PCR System (Thermo Fisher Scientific, Singapore) and analyzed with QuantStudio 3D AnalysisSuite software (v1.1, Thermo Fisher Scientific, Carlsbad, CA, USA).

    Article Title: Enhanced CRISPR/Cas-Based Immunoassay through Magnetic Proximity Extension and Detection
    Article Snippet: .. To perform digital magnetic PEA RPA-CRISPR/Cas12a-assisted immunoassay in QuantStudio digital chips, assembled digital RPA-CRISPR/Cas12a-assisted mix was first loaded into a commercial QuantStudio 3D Digital PCR 20K Chip v2 (ThermoFisher Scientific, Waltham, MA) by using a QuantStudio 3D Digital PCR Chip Loader and closely following the user guide. ..

    Article Title: Monitoring SARS-CoV-2 RNA in Wastewater with RT-qPCR and Chip-Based RT-dPCR: Sewershed-Level Trends and Relationships to COVID-19
    Article Snippet: The RT-dPCR reaction mixture was composed of 7.5 μL of QuantStudio 3D Digital PCR Mastermix v2 (Applied Biosystems), 500 nM each of forward and reverse primers, 125 nM probes, 3 μL of cDNA template, and nuclease free water to bring the total volume to 15 μL. .. 14.5 μL of reaction mixture was loaded onto a chip using the QuantStudio 3D Digital PCR Chip Loader (Applied Biosystems, Catalog No. 4482592) according to manufacturer instructions. ..

    Article Title: CASMART, a one-step CRISPR Cas12a-mediated isothermal amplification for rapid and high-resolution digital detection of rare mutant alleles.
    Article Snippet: Convenient, ultrasensitive, and accurate detection of rare variants is essential for early cancer diagnosis and precision medicine, however, despite years of efforts, tools that have all these qualities remain elusive.. Here, we developed a one-step CRISPR/Cas12a-based digital diagnostic platform for accurately quantifying mutant alleles, referred to as the CRISPR ASsoaciated Mutation Allele Rapid Test (CASMART).. The platform accurately quantifies the variant allele frequency of EGFR L858R within 1 h at 42 C and can detect mutant targets as low as 0.3 copies/μL (0.498 aM) in mock multiplex cfDNA samples.

    Article Title: Pravastatin prevents colitis-associated carcinogenesis by reducing CX3CR1 high M2-like fibrocyte counts in the inflamed colon
    Article Snippet: .. Digital PCR was performed using a QuantStudio 3D Digital PCR System platform consisting of a QuantStudio 3D Instrument, a Dual Flat Block GeneAmp PCR System 9,700, and a QuantStudio 3D Digital PCR Chip Loader (all from Thermo Fisher Scientific). ..

    Blocking Assay:

    Article Title: Extraction-Free Absolute Quantification of Circulating miRNAs by Chip-Based Digital PCR
    Article Snippet: .. Chip-based dPCR was performed on a QuantStudio 3D Digital PCR System platform composed of the QuantStudio 3D Instrument (Thermo Fisher Scientific; 4489084), the Dual Flat Block GeneAmp PCR System 9700 (Thermo Fisher Scientific; 4484078), and the QuantStudio 3D Digital PCR Chip Loader (Thermo Fisher Scientific; 4482592). dPCR was performed according to the manufacturer’s instructions. ..

    Article Title: Pravastatin prevents colitis-associated carcinogenesis by reducing CX3CR1 high M2-like fibrocyte counts in the inflamed colon
    Article Snippet: .. Digital PCR was performed using a QuantStudio 3D Digital PCR System platform consisting of a QuantStudio 3D Instrument, a Dual Flat Block GeneAmp PCR System 9,700, and a QuantStudio 3D Digital PCR Chip Loader (all from Thermo Fisher Scientific). ..

    Polymerase Chain Reaction:

    Article Title: Extraction-Free Absolute Quantification of Circulating miRNAs by Chip-Based Digital PCR
    Article Snippet: .. Chip-based dPCR was performed on a QuantStudio 3D Digital PCR System platform composed of the QuantStudio 3D Instrument (Thermo Fisher Scientific; 4489084), the Dual Flat Block GeneAmp PCR System 9700 (Thermo Fisher Scientific; 4484078), and the QuantStudio 3D Digital PCR Chip Loader (Thermo Fisher Scientific; 4482592). dPCR was performed according to the manufacturer’s instructions. ..

    Article Title: Mitochondrial aggregation caused by cytochalasin B compromises the efficiency and safety of three-parent embryo.
    Article Snippet: .. The sample was loaded using the QuantStudio 3D Digital PCR Chip Loader (4482592, Thermo Fisher Scientific, Singapore) and amplified in a GeneAmp PCR System 9700. .. Images of chips were taken using the QuantStudio 3D Digital PCR System (Thermo Fisher Scientific, Singapore) and analyzed with QuantStudio 3D AnalysisSuite software (v1.1, Thermo Fisher Scientific, Carlsbad, CA, USA).

    Article Title: Pravastatin prevents colitis-associated carcinogenesis by reducing CX3CR1 high M2-like fibrocyte counts in the inflamed colon
    Article Snippet: .. Digital PCR was performed using a QuantStudio 3D Digital PCR System platform consisting of a QuantStudio 3D Instrument, a Dual Flat Block GeneAmp PCR System 9,700, and a QuantStudio 3D Digital PCR Chip Loader (all from Thermo Fisher Scientific). ..

    Amplification:

    Article Title: Mitochondrial aggregation caused by cytochalasin B compromises the efficiency and safety of three-parent embryo.
    Article Snippet: .. The sample was loaded using the QuantStudio 3D Digital PCR Chip Loader (4482592, Thermo Fisher Scientific, Singapore) and amplified in a GeneAmp PCR System 9700. .. Images of chips were taken using the QuantStudio 3D Digital PCR System (Thermo Fisher Scientific, Singapore) and analyzed with QuantStudio 3D AnalysisSuite software (v1.1, Thermo Fisher Scientific, Carlsbad, CA, USA).

    other:

    Article Title: Rebuilding A Collapsed Bivalve Population, Restoring Seagrass Meadows, and Eradicating Harmful Algal Blooms In A Temperate Lagoon Using Spawner Sanctuaries
    Article Snippet: The dPCR reactions were loaded onto QuantStudioTM 3D Digital PCR Chip V2 chips containing 20,000 well partitioning with the QuantStudioTM 3D Digital PCR Chip loader (Applied Biosystems, Foster City, CA, USA), sealed with immersion fluid and the chip lid per the manufacturer’s instructions.

    Recombinase Polymerase Amplification:

    Article Title: Enhanced CRISPR/Cas-Based Immunoassay through Magnetic Proximity Extension and Detection
    Article Snippet: .. To perform digital magnetic PEA RPA-CRISPR/Cas12a-assisted immunoassay in QuantStudio digital chips, assembled digital RPA-CRISPR/Cas12a-assisted mix was first loaded into a commercial QuantStudio 3D Digital PCR 20K Chip v2 (ThermoFisher Scientific, Waltham, MA) by using a QuantStudio 3D Digital PCR Chip Loader and closely following the user guide. ..



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    Validation of miRNA expression by <t>qRT-PCR</t> and <t>digital</t> <t>PCR</t> assays. qRT-PCR miRNA expression assays of GT1–7 cells and exosomes from differential UC (UCexo) and Optiprep fractions 7, 8, 9 and 10 for (A) let-7b and (B) miR-342-3p, data expressed as raw Ct values, n = 3. Digital PCR of miRNA in GT1–7 cells and exosomes from differential UC (UCexo) and Optiprep fractions 7, 8, 9 and 10 for let-7b (C) and miR-342-3p (D), data expressed as copies per μL, n = 3. Standard deviations are shown in the graph.
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    <t> dPCR </t> identification of lingonberry and cranberry DNA in mixed DNA samples.
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    Image Search Results


    Validation of miRNA expression by qRT-PCR and digital PCR assays. qRT-PCR miRNA expression assays of GT1–7 cells and exosomes from differential UC (UCexo) and Optiprep fractions 7, 8, 9 and 10 for (A) let-7b and (B) miR-342-3p, data expressed as raw Ct values, n = 3. Digital PCR of miRNA in GT1–7 cells and exosomes from differential UC (UCexo) and Optiprep fractions 7, 8, 9 and 10 for let-7b (C) and miR-342-3p (D), data expressed as copies per μL, n = 3. Standard deviations are shown in the graph.

    Journal: RNA Biology

    Article Title: Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery

    doi: 10.1080/15476286.2016.1270005

    Figure Lengend Snippet: Validation of miRNA expression by qRT-PCR and digital PCR assays. qRT-PCR miRNA expression assays of GT1–7 cells and exosomes from differential UC (UCexo) and Optiprep fractions 7, 8, 9 and 10 for (A) let-7b and (B) miR-342-3p, data expressed as raw Ct values, n = 3. Digital PCR of miRNA in GT1–7 cells and exosomes from differential UC (UCexo) and Optiprep fractions 7, 8, 9 and 10 for let-7b (C) and miR-342-3p (D), data expressed as copies per μL, n = 3. Standard deviations are shown in the graph.

    Article Snippet: For digital PCR, cDNA was diluted 1:10 and set-up in duplicate reactions containing the specific TaqMan® miRNA assay and digital PCR master mix which was subsequently loaded and sealed into a QuantStudio™ 3D Digital PCR 20K Chip using QuantStudio™ 3D Digital PCR Chip Loader (Applied Biosystems), according to the manufacturer's instructions.

    Techniques: Expressing, Quantitative RT-PCR, Digital PCR

     dPCR  identification of lingonberry and cranberry DNA in mixed DNA samples.

    Journal: Foods

    Article Title: A dPCR Method for Quantitative Authentication of Wild Lingonberry ( Vaccinium vitis-idaea ) versus Cultivated American Cranberry ( V. macrocarpon )

    doi: 10.3390/foods11101476

    Figure Lengend Snippet: dPCR identification of lingonberry and cranberry DNA in mixed DNA samples.

    Article Snippet: The reaction mixture was immediately loaded onto chips using a QuantStudio TM 3D Digital PCR Chip Loader (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: